题目：Microbioreactors and 3D Fluorescent Cell-Based High-Throughput Screening (HTS) for Drug Discovery
报告人：Shang-Tian Yang, Professor
摘要 :To accelerate the discovery of chemicals with biological activities, we have developed fluorescence-based live 3D cell cultures in microwell plates and microfluidic biochips for high-throughput cytotoxicity and proliferation assays using enhanced green fluorescent protein (EGFP)-expressing cells. This is the first cell-based high-throughput system in 3D that can mimic in vivo environment and be used reliably for screening chemicals and biomolecules as potential drug targets. Drug responses of the cells in this 3D system can be monitored in real time and noninvasively. The microbioreactor arrays can also be used for medium optimization and cell culture process development, and thus will have wide applications in drug discovery, cell culture process development, and tissue engineering. This presentation will outline the principles and methods used in this 3D HTS system and provide several application examples in cytotoxicity and embryotoxicity assays, drug efficacy study, and biomolecule discovery, particularly in screening herbal medicines for potential effects on stimulating stem cell growth and/or inhibiting cancer cells.
简介：Dr. Yang is Professor of Chemical and Biomolecular Engineering, Food Science and Technology, Biomedical Engineering, and Molecular Genetics at the Ohio State University, where he has been on the faculty since 1985. He is also the director of Ohio Bioprocessing Research Consortium and has worked with many companies in commercial technology development. Dr. Yang received his B.S. degree in Agricultural Chemistry from National Taiwan University and M.S. and Ph.D. degrees in Biochemical Engineering from Purdue University. Dr. Yang has broad research interests in bioengineering. His current research involves biocatalysis, metabolic engineering, cell culture, tissue engineering, functional genomics, and microfluidic biochips for high-throughput cell-based assays and biodiagnostics. He has more than 280 scientific publications and 12 patents in the bioengineering field. Dr. Yang is also a co-founder of two biotechnology startup companies. He is an elected fellow of American Institute of Medical and Biological Engineering, Associate Editor for the journal Process Biochemistry, past chair of Division 15 Food, Pharmaceutical and Bioengineering of American Institute of Chemical Engineers (AIChE) and an active member of American Chemical Society (ACS).